Effect of Hydroalcoholic Extract of Ephedra major, Momordica charantia, and Resveratrol on Cytotoxicity and Caspase-3 Genes Expression Level in MCF-7 Breast Cancer Cell Line

Background: To increase the therapeutic effect of drugs to combat diseases, combination therapy with current chemical and new medicines derived from medicinal plants is necessary. Objectives: The present work aimed investigate hydroalcoholic extract two plants, Ephedra major Momordica cacharantia (Carla), resveratrol drug on cell viability expression levels caspase-3 gene in MCF-7 line. Methods: In this experimental study, extraction tested was done a Soxhlet extractor. MTT assay real-time PCR were used determine toxicity levels, respectively. Results: highest lowest cytotoxic effects plant extracts observed at concentrations 500 150 µg/mL, level after 72 h incubation by different resveratrol. Conclusions: It can be concluded that both could influence cells via gene. Thus, these species pharmaceutical industry.